Search results for "CNS development"

showing 3 items of 3 documents

The dynamic properties of neuronal chromatin are modulated by triiodothyronine.

1992

The effect of triiodothyronine (T3) on the rate of synthesis of nuclear proteins was studied during terminal differentiation of rat cortical neurons cultured in a serum-free medium. To this aim total and acid soluble nuclear proteins were analyzed by different electrophoretic techniques. Our results show that: 1) during maturation in vitro, neuronal nuclei undergo a dramatic change in the rate at which different classes of histones and high mobility group (HMG) proteins are synthesized; the synthetic activity, measured as incorporation of radioactive precursors into nuclear proteins, slows indeed down with age: especially evident is the decrease in core histones synthesis; at day 15, on the…

CNS developmentLysineBiologyBiochemistryCellular and Molecular NeuroscienceSettore BIO/10 - BiochimicamedicineAnimalsSettore BIO/06 - Anatomia Comparata E CitologiaNuclear proteinCells CulturedNeuronsTriiodothyronineLysineGeneral MedicineneuronChromatinChromatinCell biologyRatsCell nucleusmedicine.anatomical_structureHigh-mobility groupHistoneBiochemistrySolubilitybiology.proteinTriiodothyronineSettore MED/26 - NeurologiaElectrophoresis Polyacrylamide GelNeuronNeurochemical research
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Abdominal-B and caudal inhibit the formation of specific neuroblasts in the Drosophila tail region

2013

The central nervous system of Drosophila melanogaster consists of fused segmental units (neuromeres), each generated by a characteristic number of neural stem cells (neuroblasts). In the embryo, thoracic and anterior abdominal neuromeres are almost equally sized and formed by repetitive sets of neuroblasts, whereas the terminal abdominal neuromeres are generated by significantly smaller populations of progenitor cells. Here we investigated the role of the Hox gene Abdominal-B in shaping the terminal neuromeres. We show that the regulatory isoform of Abdominal-B (Abd-B.r) not only confers abdominal fate to specific neuroblasts (e.g. NB6-4) and regulates programmed cell death of several proge…

Central Nervous SystemTailanimal structuresCNS developmentCellular differentiationParaHoxApoptosisBiologyTerminal neuromeresAbdominal-BHox genesNeural Stem CellsNeuroblastNeuroblastsImage Processing Computer-AssistedAnimalsDrosophila ProteinsHox geneMolecular BiologyIn Situ HybridizationDNA PrimersHomeodomain ProteinsfungiCell DifferentiationStem Cells and RegenerationNeuromereImmunohistochemistryMolecular biologyNeural stem cellSegmental patterningDrosophila melanogasterMicroscopy Fluorescencenervous systemembryonic structuresCaudalDrosophilaGanglion mother cellDrosophila ProteinTranscription FactorsDevelopmental BiologyDevelopment
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Triiodothyronine accelerates the synthesis of synapsin I in developing neurons from fetal rat brain cultured in a synthetic medium.

1990

The effect of Triiodothyronine (T3) on Synapsin I appearance in rat cortical neurons has been investigated in vitro. Neuronal cultures from 16-day-old fetal rat brain grown in the absence of T3, express immunohystochemically detectable Synapsin I at the 14th day in vitro. The addition of the hormone to the culture medium determines an early (at the 7th day in vitro) appearance of fluorescent dots specific for Synapsin I. © 1990 Plenum Publishing Corporation.

Synapsin Imedicine.medical_specialtyCNS developmentCentral nervous systemFluorescent Antibody TechniqueNerve Tissue ProteinsBiologySettore BIO/19 - Microbiologia GeneraleBiochemistryCellular and Molecular NeuroscienceFetusInternal medicinemedicineAnimalschemically defined mediumimmunofluorescenceCells CulturedNeuronsFetusTriiodothyronineNeuroscience (all)BrainGeneral MedicineSynapsinsIn vitroCulture MediaRatsChemically defined mediummedicine.anatomical_structureEndocrinologynervous systemneuronal cultureCerebral cortexCell cultureTriiodothyronineSettore MED/26 - NeurologiaSynapsin 1Neurochemical research
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